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目的:建立氯仿萃取-分光光度法测定β-胡萝卜素水溶性制剂中β-胡萝卜素的含量方法.方法:将样品经氯仿分步萃取后,以环己烷为溶剂,在455±1nm波长处,用分光光度法测定A值.结果β-胡萝卜素浓度在1. 60～3.20μg·ml~(-1)时,吸收值与浓度呈线性关系,相关系数(r=0.9998,平均回收率为99.9%,RSD为0.74%(n= 5.结论:本方法可行,测定结果准确,精密度,适用于β-胡萝卜素水溶性制剂中β-胡萝卜素的含量测定.
OBJECTIVE: To establish a method for the determination of β-carotene content in β-carotene water-soluble preparations by chloroform extraction-spectrophotometry.METHODS: The sample was extracted by chloroform and then treated with cyclohexane as solvent at a wavelength of 455 ± 1nm , And the value of A was determined by spectrophotometry.Results When the concentration of β-carotene was 1.60 ~ 3.20μg · ml -1, the correlation coefficient (r = 0.9998, the average recovery was 99.9%, RSD 0.74% (n = 5) .Conclusion: The method is feasible, accurate and accurate, suitable for the determination of β-carotene content in β-carotene water-soluble preparation.