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目的 :研究异种单个核细胞在机体内的分布及定植特点 ,为细胞疫苗的研究和改进提供理论基础。方法 :经兔外周动、静脉输入99mTC标记处理和未处理的人单个核细胞 ,用单光子发射计算机断层仪(Single photonemissioncomputedtomography,SPECT)扫描并做图像分析及用γ 记数仪测定放射量。结果 :异种单个核细胞主要定植在肺、肝、脾及骨髓 ,而胸腺、淋巴结、肌肉、肠壁等其它脏器定植极少 ;不同途径输入的异种单个核细胞对以上分布特点无明显影响 ,而不同的预处理对异种单个核细胞的分布特点有影响。结论 :异种单个核细胞在体内的定植具有一定的组织器官特异性 ,不同脏器血管内皮细胞对“扣押”异种细胞的机制可能不同。
OBJECTIVE: To study the distribution and colonization characteristics of xenogenic mononuclear cells in vivo and to provide a theoretical basis for the research and improvement of cellular vaccines. METHODS: 99mTC-labeled and untreated human mononuclear cells were injected into the peripheral arterial and venous branches of rabbits. The lesions were scanned by single photonemission computed tomography (SPECT) and analyzed by image analysis. The radioactivity was measured by gamma-counting. Results: Allogeneic mononuclear cells were colonized mainly in lung, liver, spleen and bone marrow, while those in other organs such as thymus, lymph node, muscle and intestine wall were rarely colonized. The heterologous mononuclear cells input by different pathways had no obvious effect on the above distribution characteristics, The different pretreatments on the distribution of heterogeneous mononuclear cells have an impact. Conclusion: The establishment of xenogeneic mononuclear cells in vivo has certain tissue-organ specificity. The mechanism of “detaining” xenogeneic cells by vascular endothelial cells of different organs may be different.