论文部分内容阅读
绿脓杆菌是肺囊肿纤维化病人的主要肺部感染菌。研究表明菌体表面的粘液样胞外多糖(MEP)成份是引起持续感染的重要因素。针对MEP的调理性抗体具有一定的免疫保护效果,而非调理性抗体则无,且MEP在体内大多数只产生非调理性抗体这一缺点,作者构建了一株特殊的抗独特型单克隆抗体。方法是用临床分离的绿脓杆菌2192株免疫小鼠,按标准方法构建杂交瘤,筛选出具有免疫调理活性的抗MEP单克隆抗体McAb1;然后用McAb 1免疫BALB/cBy(?)小鼠,待抗F(ab’)_2片段抗体出现后取其脾细
Pseudomonas aeruginosa is a major lung infection in patients with cystic fibrosis. Studies have shown that mycobacterial mucosal polysaccharide (MEP) component of the cell surface is an important factor in causing persistent infection. The conditioning antibodies against MEP have some immunoprotective effects, whereas the non-conditioning antibodies are absent, and MEP produces only non-conditioning antibodies in vivo. The authors constructed a unique anti-idiotypic monoclonal antibody . The method comprises the following steps: immunizing mice with clinical isolates of Pseudomonas aeruginosa 2192, constructing hybridoma according to the standard method, screening anti-MEP monoclonal antibody McAb1 with immunomodulatory activity; and then immunizing BALB / cBy mice with McAb 1, To be anti-F (ab ’) _ 2 fragment antibodies take splenomegaly