论文部分内容阅读
种质资源研究是育种工作的基础,关联分析为植物数量性状的研究提供了有力手段。本研究利用67对EST-SSR标记,对115份茶树(Camellia sinensis)资源进行茶品质相关性状与EST-SSR标记的关联分析。基因间的连锁不平衡是关联分析的基础,67对EST-SSR组成的共2 211对成对位点组合中,均存在一定程度的连锁不平衡,由群体结构分析发现,当群体数目K=4时,模型后验概率Ln P(D)最大,该群体被分为4个亚群。将各个材料的Q值作为协变量,分别进行4种目标性状的变异与EST-SSR标记变异的回归分析。由结果得知,与水浸出物、茶多酚、氨基酸和咖啡碱关联的标记共19个,其中与水浸出物关联的标记有5个,TM066对其解释率最高,为13.32%;与茶多酚关联的标记有5个,TM092对其解释率最高,为13.68%;与氨基酸关联的标记有6个,TM083对其解释率最高,为7.6%;与咖啡碱关联的标记有3个,TM111对其解释率最高,为7.8%。其中TM066、TM092和TM074-2与水浸出物和茶多酚同时关联;TM086-1与茶多酚和氨基酸同时关联;TM088、TM111和TM124与氨基酸和咖啡碱同时关联。这些研究结果可进一步用于相关性状位点的深入研究,为茶树的早期鉴定和茶叶品质的改良等提供一定的理论参考。
Germplasm resources research is the basis of breeding work, and the correlation analysis provides a powerful tool for the study of plant quantitative traits. In this study, 67 pairs of EST-SSR markers were used to analyze the correlation between tea quality-related traits and EST-SSR markers in 115 Camellia sinensis resources. The linkage disequilibrium among genes was the basis of correlation analysis. A total of 2 211 pairs of pairs of EST-SSR pairs consisted of a pair of linkage disequilibrium. According to the analysis of population structure, it was found that when the population number K = 4, the model posterior probability Ln P (D) is the largest, the population is divided into four subgroups. The Q value of each material as a covariate, respectively, four kinds of traits of variation and EST-SSR marker regression analysis. According to the results, there were 19 markers associated with water extracts, tea polyphenols, amino acids and caffeine, of which 5 were associated with water extracts. TM066 had the highest interpretation rate of 13.32% TM092 had the highest rate of interpretation of 13.68%, 6 of 6 markers were associated with amino acids, TM083 had the highest rate of interpretation, accounting for 7.6%, 3 markers associated with caffeine, TM111 The highest rate of explanation, 7.8%. Among them, TM066, TM092 and TM074-2 were correlated with water extracts and tea polyphenols simultaneously; TM086-1 was associated with tea polyphenols and amino acids simultaneously; TM088, TM111 and TM124 were simultaneously associated with amino acids and caffeine. These results can be further used for further research on the trait loci, providing some theoretical references for the early identification of tea plants and the improvement of tea quality.