液体菌种的保存适温及其有效期,是它能否大面积应用的关键之一。为此,我们对液体菌种作了在不同温度下的有效期试验,试图为液体菌种保存、使用提供可靠依据。现将试验结果介绍如下:一、材料和方法(一)供试菌种;香菇洞K 菌株(引自山东外贸局),扩繁后经500立升不诱钢标准发酵罐培养94小时,菌球(片)数为1026个/毫升,菌丝干重达5.4克/100毫升。 Liquid spawn temperature preservation and its validity, it is one of the keys to whether it can be applied in large area. To this end, we made liquid bacteria at different temperatures of the validity of the trial, trying to save the liquid bacteria used to provide a reliable basis. Now the test results are introduced as follows: First, the material and methods (A) for the test species; Mushroom hole K strain (cited from the Shandong Foreign Trade Bureau), after the proliferation of 500 liters of stainless steel fermentation standard fermentor training 94 hours, The number of balls (slices) was 1026 / ml, and the mycelium dry weight reached 5.4 g / 100 ml.