我们检测急性川崎病(Kawasaki disease,KD)患儿外周血中淋巴细胞凋亡和Bcl-2基因表达,以探讨其与KD发病的关系。 对象与方法 1 研究对象 住院急性KD患儿10例,男8例,女2例,年龄10月至3岁9月,均符合日本川崎病研究会诊断标准。对照组健康儿童8例,男6例,女2例,年龄1～5岁。 2 方法 ①标本采集和制备:健康儿童和患儿用药前及用药后(阿斯匹林50mg/kg.d,热 We detected lymphocyte apoptosis and Bcl-2 gene expression in peripheral blood of children with acute Kawasaki disease (KD) to investigate their association with the pathogenesis of KD. Subjects and methods A study of 10 cases of acute KD hospitalized children, 8 males and 2 females, aged from October to 3 years of age, are in line with the Japanese Kawasaki Disease Research Association diagnostic criteria. Control group, 8 healthy children, 6 males and 2 females, aged 1 to 5 years old. 2 Methods ① specimen collection and preparation: healthy children and children before medication and after treatment (aspirin 50mg / kg.d, heat