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目的:研究利心冲剂对心衰大鼠治疗作用及药物毒理学。方法:实验分组:正常对照组、模型组、利心冲剂低剂量组、利心冲剂高剂量组、卡托普利阳性药组。腹腔注射阿霉素(ADR)5mg·kg-1,每5d1次,共3次,复制大鼠心衰模型,左心室插管术测定血流动力学指标,HE病理染色;毒理学研究测定大鼠LD50和最大耐受量,另予利心冲剂4,8,16g·(100g)-13个剂量组和空白对照组,连续8周口服给药,测定大鼠体重,于停药时及停药后7d进行血液常规、肝功能、肾功能检查,实验结束处死动物,计算脏器系数,进行病理学检验。结果:利心冲剂治疗组和模型组比较,左心室收缩内压显著升高,形态学显示心肌细胞变性坏死明显减轻;毒性试验表明,以成人口服剂量的60倍经口灌胃,未见大鼠明显毒性反应;长期应用对大鼠体重、血常规、肝功能、肾功能和脏器系数、病理均无影响。结论:利心冲剂能改善心脏功能,具有较高的安全性。
Objective: To study the therapeutic effects and drug toxicology of Lixin granule on heart failure rats. Methods: Experimental grouping: normal control group, model group, low-dose group of Lixin Granules, high-dose group of Lixin Granules, and captopril-positive group. Intraperitoneal injection of adriamycin (ADR) 5mg·kg-1, every 5d 1 times, total 3 times, replicate rat heart failure model, left ventricular catheterization to measure hemodynamic parameters, HE pathological staining; toxicology studies measuring large Rat LD50 and maximal tolerated doses were given separately to Lixin granules 4,8,16g·(100g)-13 dose groups and blank control group. Oral administration was continued for 8 weeks. Rat body weight was measured and stopped when stopped. Blood routine, liver function, and kidney function tests were performed 7 days after drug administration. Animals were sacrificed at the end of the experiment, and the organ coefficient was calculated and examined for pathology. RESULTS: Compared with the model group, the xinxin granules treatment group’s systolic pressure increased significantly, and the morphology showed that the degeneration and necrosis of cardiomyocytes was significantly reduced; the toxicity test showed that 60 times the oral dose of adult oral gavage, no large Obvious toxicity in rats; long-term application of weight, blood routine, liver function, renal function and organ coefficient, pathology had no effect. Conclusion: Lixin Granules can improve heart function and have higher safety.