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目的探讨依那普利对丙烯醛吸入致大鼠炎性损伤肺组织中IL-1β、IL-6表达变化的影响。方法将大鼠随机分为4组:生理盐水雾化吸入组,丙烯醛雾化吸入组,依那普利治疗组,依那普利自身对照组。用丙烯醛雾化吸入造成大鼠气道黏液高分泌炎性气道肺损伤模型。于造模及依那普利处理后1、3、6周分别用免疫组织化学染色、原位杂交、RT-PCR和蛋白免疫印迹技术检测肺组织IL-1β和IL-6蛋白及mRNA的定位与表达量。结果 IL-1β、IL-6主要分布于肺上皮细胞,胞浆染色为主。丙烯醛吸入后3周,肺组织炎症反应明显,IL-1β和IL-6的mRNA和蛋白水平均明显上调,6周达到高峰(P<0.05),而依那普利治疗下调IL-1β和IL-6mRNA和蛋白水平(P<0.05)。结论依那普利的抗炎作用可能与IL-1β和IL-6的表达下调有关。
Objective To investigate the effect of enalapril on the expression of IL-1β and IL-6 in lung tissue of rats with inflammatory injury induced by acrolein. Methods The rats were randomly divided into 4 groups: saline inhalation group, acrolein inhalation group, enalapril treatment group, enalapril self-control group. Inhalation of acrolein induced airway mucus hypersecretion inflammatory airway injury model in rats. Lung tissue IL-1β and IL-6 protein and mRNA were detected by immunohistochemical staining, in situ hybridization, RT-PCR and Western blotting 1, 3 and 6 weeks after model and enalapril treatment With the amount of expression. Results IL-1β and IL-6 mainly distributed in lung epithelial cells, and cytoplasm staining was dominant. Three weeks after the inhalation of acrolein, the inflammation of the lung tissue was obvious. The mRNA and protein levels of IL-1β and IL-6 were significantly increased (P <0.05) at 6 weeks, while the levels of IL-1β IL-6 mRNA and protein levels (P <0.05). Conclusion The anti-inflammatory effect of enalapril may be related to the down-regulation of IL-1β and IL-6.