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目的采用自行设计的小动物呼气试验模型进行大鼠L-[1-13C]苯丙氨酸呼气试验(13C- PheBT),以验证该实验方法的可行性和有效性,并提供有效的试验参数。方法280～290g的雄性SD 大鼠20只,随机分为急性肝损伤组和正常对照组,每组10只,采用四氯化碳橄榄油灌胃染毒复制急性肝损伤模型,小动物呼气机进行机械通气制作呼气试验模型;按20mg／kg体重尾静脉弹丸给予13C- 苯丙氨酸(13C-Phe),收集给药前和给药后1～60min呼出气样共29次气样,应用气体同位素比值质谱仪测定样品中13C丰度。结果13C排除时相曲线呈单峰,峰值多位于给药后2min;急性肝损伤大鼠呼气试验参数13C排除速率常数(PheBT-K)为(2．45±0．25)×10-2min-1,显著低于正常对照组(2．98±0．19) ×10-2min-1(t=5．40,P<0．001),而急性肝损伤大鼠呼出气中13C排除峰值和血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST、总胆汁酸(TBA)、碱性磷酸酶(AKP)和总胆红素(TBIL)含量均显著高于正常对照组(t值分别为8．15,3．40,3．90,4．83和4．12,P<0．05);13C快处置常数在两组间差异无显著性(t= 0．58,P>O．05);急性肝损伤大鼠PheBT-K与血清ALT和AKP活度以及TBA和TBIL水平呈负相关(r 分别为-0．74、-0.73、-0．82和-0．67,P值均小于O．05),而与血清AST活度无相关性(r=0．16,p> 0．05)。结论自行设计的小动物呼气试验模型是进行呼气试验基础研究的有效工具;动物PheBT-K 是一项灵敏的分析指标。
OBJECTIVE: To investigate the feasibility and effectiveness of this experimental method using the designed animal breath test model for rat L- [1-13C] phenylalanine breath test (13C-PheBT), and to provide an effective Test parameters. Methods Twenty male SD rats (280 ~ 290g) were randomly divided into acute liver injury group (n = 10) and normal control group (n = 10). The rats were treated with carbon tetrachloride olive oil The mechanical ventilation was used to make the breath test model. 13C-Phe was administered to the caudal vein progenitor at a dose of 20 mg / kg body weight, and gas samples were exhaled before and after 1 ~ 60 min , 13C abundance in the sample was measured using a gas isotope ratio mass spectrometer. Results The 13C exclusion phase curve showed a single peak with the peak value at 2min after administration. The 13C elimination rate constant (PheBT-K) of the expiratory parameters in acute liver injury rats was (2.45 ± 0.25) × 10-2min -1, significantly lower than that of the control group (2.98 ± 0.19) × 10-2min-1 (t = 5.40, P <0.001) (ALT), aspartate aminotransferase (AST), total bile acid (TBA), alkaline phosphatase (AKP) and total bilirubin (TBIL) in serum were significantly higher than those in the normal control group t values were 8.15, 3.40, 3.90, 4.83 and 4.12 respectively, P <0.05). There was no significant difference between the two groups in the 13C fast disposal parameters (t = 0.58, P> 0.05). PheBT-K in acute liver injury rats was negatively correlated with serum ALT and AKP activities, TBA and TBIL levels (r = -0.74, -0.73, -0.82 and -0.0, respectively) 67, P values were less than 0.05), but no correlation with serum AST activity (r = 0.16, p> 0.05) .Conclusion The self-designed small animal breath test model is the basis of breath test An effective tool for research; animal PheBT-K is a sensitive indicator of analysis.