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目的 摸索可靠、高效和特异的聚合酶链反应 (PCR)条件 ,分析多肿瘤抑癌基因 1(MTS1)第二外显子纯合性缺失。方法 对一系列PCR参数进行摸索同时设立对照 ,并对PCR产物进行Southernblot分析。结果 PCR产物是特异的MTS1第二外显子。结论 扩增MTS1第二外显子的PCR反应条件可以得到可靠、特异的结果。
Objective To explore reliable, efficient, and specific polymerase chain reaction (PCR) conditions and analyze the homozygous deletion of the second exon of the multiple tumor suppressor gene 1 (MTS1). Methods A series of PCR parameters were explored and a control was set up. The PCR products were analyzed by Southern blot. Results The PCR product was specific to the second exon of MTS1. Conclusion The amplification of MTS1 exon 2 PCR reaction conditions can obtain reliable and specific results.