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目的:观察肉苁蓉苯乙醇苷预防给药对高原肺水肿模型大鼠的作用及可能的作用机制。方法:正常对照组,模型组,大花红景天口服液组(1.78 mL·kg-1·d-1),肉苁蓉苯乙醇苷低、中、高剂量组(75,150,300 mg·kg-1·d-1),每组12只,灌胃预防给药10 d,第8天起除空白对照组外其余各组置于模拟海拔5 000 m高原环境72 h建立高原肺水肿模型,观察各组大鼠肺组织病理改变,检测肺组织含水量、肺组织匀浆中白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、丙二醛(MDA)含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)酶活力。结果:与正常对照组[肺组织含水量为(77.38±0.67)%,肺匀浆IL-6含量(181.49±26.00)ng·L-1,TNF-α含量(83.33±33.31)ng·L-1,MDA含量(5.25±1.13)nmol·mg-1,SOD酶活力(124.74±35.45)U·mg-1,GSH-Px酶活力(234.16±17.33)U·mg-1]相比,模型组大鼠出现明显的肺水肿,肺组织含水量显著升高、肺匀浆液中IL-6,TNF-α,MDA含量增高,SOD和GSH-Px酶活力显著降低。与模型组比较,肉苁蓉苯乙醇苷能够改善高原肺水肿大鼠肺水肿病理改变,降低肺组织含水量,降低肺匀浆中IL-6,TNF-α,MDA含量且提高SOD和GSH-Px的酶活力。结论:肉苁蓉苯乙醇苷能够预防高原肺水肿的发生,与其抗炎、抗氧化应激有关。
Objective: To observe the effect and the possible mechanism of preventive effect of Cistancin acetate on rats with high altitude pulmonary edema. Methods: The normal control group, model group, Dahua Hongjingtian oral liquid group (1.78 mL · kg-1 · d-1), Cistanche etongalide low, middle and high dose group (75,150,300 mg · kg-1 · d- 1), 12 rats in each group were given gavage for 10 days. From the 8th day, except the blank control group, the other groups were set up in a simulated altitude of 5000 m plateau for 72 hours to establish the model of high altitude pulmonary edema. The rats in each group were observed The pathological changes of lung tissue were observed. The water content of lung tissue, contents of IL-6, TNF-α and MDA in lung tissue homogenate and the content of superoxide dismutase (SOD) SOD), glutathione peroxidase (GSH-Px) enzyme activity. Results: Compared with the normal control group [the water content in lung tissue was (77.38 ± 0.67)%, IL-6 content in lung homogenate was (181.49 ± 26.00) ng · L-1 and the content of TNF-α was 83.33 ± 33.31 ng · L- 1, MDA content (5.25 ± 1.13) nmol · mg-1, SOD activity (124.74 ± 35.45) U · mg-1, GSH-Px enzyme activity (234.16 ± 17.33) U · mg-1] Significant pulmonary edema occurred in rats, and the water content in lung tissue was significantly increased. The content of IL-6, TNF-α and MDA in lung homogenate increased significantly and the activity of SOD and GSH-Px decreased significantly. Compared with the model group, Cistanche Phenethoside can improve pathological changes of pulmonary edema, reduce the water content of lung tissue, decrease the content of IL-6, TNF-α and MDA in lung homogenate and increase the content of SOD and GSH-Px Enzyme activity. CONCLUSION: Cistanche Phenethol can prevent the occurrence of high altitude pulmonary edema, which is related to its anti-inflammatory and anti-oxidative stress.