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目的 :用BALB/c小鼠建立自身免疫性心肌炎动物模型。方法 :从新鲜猪心中分离提纯心肌肌凝蛋白。在第 1,第 8和第 3 0天用心肌肌凝蛋白和完全弗氏佐剂混合的乳浊液皮下注射免疫实验组小鼠 ,而在对照组仅用完全弗氏佐剂皮下注射。于初次免疫后的第14、第 2 1、第 3 0和第 60天收集血液和心脏标本 ,进行肌凝蛋白抗体和组织病理学检查。结果 :模型组第 14天到第 3 0天的标本中有不同程度的炎性反应和心肌细胞变性坏死 ,而 60天时出现了纤维化。同时在模型组小鼠血清中检测到肌凝蛋白抗体。结论 :通过免疫心肌肌凝蛋白发生自身免疫性心肌炎的BALB/c小鼠可作为良好的研究心肌炎和扩张型心肌病发病机制的动物模型
Objective: To establish an animal model of autoimmune myocarditis with BALB / c mice. Methods: Myocardial myosin was isolated and purified from fresh porcine heart. Immunized mice were subcutaneously injected with emulsions mixed with cardiac myosin and complete Freund’s adjuvant on days 1, 8, and 30 and mice in the control group were injected subcutaneously with complete Freund’s adjuvant alone. Blood and cardiac samples were collected on the 14th, 21st, 30th and 60th days after the initial immunization for myosin antibody and histopathological examination. Results: The model group from day 14 to day 30 had different degrees of inflammatory reaction and necrosis of cardiomyocytes in the specimens, but fibrosis occurred in 60 days. At the same time, myosin antibody was detected in the model group mice serum. CONCLUSIONS: BALB / c mice that develop autoimmune myocarditis by immunosuppressive cardiac myosin can be used as a good animal model for studying the pathogenesis of myocarditis and dilated cardiomyopathy