Hepatocellular carcinoma (HCC) is the most prominent form of presentation in liver cancer.It is also the fourth most common cause of cancer-associated deaths globally.The role of nucleus accumbens associated protein-1 (NACC-1) has been evaluated in several cancers.This protein is a transcriptional regulator that regulates a number of significant cellular processes.In the current study,we aimed to understand the role of NACC-1 in HCC.Primarily,we measured the expression of NACC-1 using quantitative real time polymerase chain reaction and weste blot analysis.We knocked down the expression of NACC-1 in HCC cell lines Huh7 and HepG2 by transferring a commercially synthesized small interfering RNA and explored the impact of NACC-1 knockdown on cellular growth,migration,invasion,and chemoresistance to doxorubicin.Through bioinformatic analysis,we identified NACC-1 as a potential target of miR-760.Using a dual reporter luciferase assay,we confirmed the predicted target and assessed miR-760-mediated regulation of NACC-1 and rescue of tumorigenic phenotypes.We observed increased expression of NACC-1 in HCC.Furthermore,knockdown of NACC-1 resulted in reduced cell proliferation and invasion and increased susceptibility to doxorubicin-mediated chemosensitivity.Overexpression of miR-760 in HCC cell lines rescued NACC-1-mediated migration and invasion.We revealed that miR-760 regulated NACC-1 expression in HCC.Our data indicated that both miR-760 and NACC-1 could be used as prognostic markers,and miR-760 may have therapeutic benefits for HCC and other cancers.