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目的:观察大蒜素对大鼠肝缺血再灌注损伤的保护作用及大蒜素对肝缺血再灌注后肝脏细胞凋亡和p53、bcl-2蛋白的影响。方法:SD大鼠,随机分为假手术组、缺血再灌注组(I/R组)和I/R加大蒜素处理组。肝脏缺血再灌注后,分别观察血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)及乳酸脱氢酶(LDH)丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性的变化;HE染色光镜下观察肝细胞的病理形态学变化;TUNEL染色观察细胞凋亡的情况;免疫组化观察肝组织bcl-2、p53蛋白含量变化。结果:经大蒜素处理后,与缺血再灌注组相比较,血清中ALT、AST、LDH、MDA含量均降低,而SOD活性增高(P<0.05);肝细胞形态学异常改变明显减轻;肝凋亡细胞减少,p53蛋白降低,bcl-2蛋白表达增加。结论:大蒜素可能通过拮抗脂质过氧化反应,清除自由基的生成,调节bcl-2、p53蛋白发挥抗凋亡作用,从而减轻缺血再灌注所造成的肝脏损伤。
Objective: To observe the protective effect of allicin on hepatic ischemia-reperfusion injury in rats and the effect of allicin on hepatic cell apoptosis and p53, bcl-2 protein after hepatic ischemia-reperfusion. Methods: SD rats were randomly divided into sham operation group, ischemia / reperfusion group (I / R group) and I / R plus allicin group. The changes of serum ALT, AST and LDH MDA and SOD activity were observed after hepatic ischemia / reperfusion. Histopathological changes of hepatocytes were observed by HE staining. Cell apoptosis was observed by TUNEL staining. The expressions of bcl-2 and p53 in liver tissue were observed by immunohistochemistry. Results: Compared with ischemia / reperfusion group, the contents of ALT, AST, LDH and MDA in serum decreased and the activity of SOD increased (P <0.05). The morphological changes of hepatocytes were significantly reduced. Apoptotic cells decreased, p53 protein decreased, bcl-2 protein expression increased. CONCLUSION: Allicin may inhibit the generation of free radicals by antagonizing lipid peroxidation and regulate the expression of bcl-2 and p53 proteins and thus reduce the hepatic injury induced by ischemia-reperfusion.