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目的构建D6S474、D20S482、D4S2408、D6S1017等4个miniSTR基因座复合扩增体系,评价其对腐败检材的应用价值,调查4个基因座在汉族人群中的遗传多态性。方法采用不同荧光标记4个miniSTR基因座上游引物,构建复合扩增体系。用分子克隆方法制备等位基因分型标准物。采用上述体系对135份汉族无关个体血样进行检测,并计算群体遗传学参数。比较该体系与ID试剂盒在降解检材分析中的成功率。结果采用本文复合扩增体系检测,汉族人群中4个基因座基因型频率分布均符合Hardy-Weinberg平衡定律,累积个人识别能力为0.999 666,累积非父排除率为0.914 902。本文体系较ID试剂盒对自然腐败检材的分型成功率更高。结论 4个miniSTR基因座复合扩增体系对法庭科学实践,特别是对腐败检材的检测有应用价值。
Objective To construct a multiplex amplification system of four miniSTR loci such as D6S474, D20S482, D4S2408 and D6S1017 to evaluate their application value to the detection of corrupt samples and to investigate the genetic polymorphism of four loci in Han population. Methods Four miniSTR loci with different fluorescent markers were used to construct a composite amplification system. Allelic typing standards were prepared by molecular cloning. Using the above system, 135 Han unrelated individual blood samples were tested, and population genetic parameters were calculated. The success rate of the system in comparison with the ID kit in degrading the sample analysis was compared. Results Using the multiplex amplification system, the genotype frequency distributions of the four loci in Han population were in accordance with the Hardy-Weinberg equilibrium law. The cumulative personal recognition ability was 0.999 666, and the cumulative non-father exclusion rate was 0.914902. This system is more successful than the ID kit in the typing of natural corrupt samples. Conclusion The 4 miniSTR loci multiplex amplification system has application value in the forensic science practice, especially in the detection of corrupt samples.