【摘 要】
：
Quinacrine, widely used to treat parasitic diseases, binds to cell membranes. We previously found that quinacrine pretreatment reduced microwave radiation damag
【机 构】
：
Department of Cognitive Sciences, Beijing Institute of Basic Medical Sciences, Beijing, China“,”Hand
论文部分内容阅读
Quinacrine, widely used to treat parasitic diseases, binds to cell membranes. We previously found that quinacrine pretreatment reduced microwave radiation damage in rat hippocampal neurons, but the molecular mechanism remains poorly understood. Considering the thermal effects of microwave radiation and the protective effects of quinacrine on heat damage in cells, we hypothesized that quinacrine would prevent microwave radiation damage to cells in a mechanism associated with cell membrane stability. To test this, we used retinoic acid to induce PC12 cells to differentiate into neuron-like cells. We then pretreated the neurons with quinacrine (20 and 40 mM) and irra-diated them with 50 mW/cm2microwaves for 3 or 6 hours. Flow cytometry, atomic force microscopy and west blot assays revealed that irradiated cells pretreated with quinacrine showed markedly less apoptosis, necrosis, and membrane damage, and greater expression of heat shock protein 70, than cells exposed to microwave irradiation alone. These results suggest that quinacrine stabilizes the neuronal membrane structure by upregulating the expression of heat shock protein 70, thus reducing neuronal injury caused by microwave radiation.
其他文献
Traumatic brain injury (TBI): Despite improved supportive and rehabilitative care of TBI patients, TBI remains a leading cause of death and disability worldwide
Recently there have been exciting research advances in neuropro-tective therapies for ischemic stroke. In the past, the search for neu-roprotective agents has b
Through complex mechanisms that guide axons to the appropriate routes towards their targets, axonal growth and guidance lead to neuronal system formation. These
This review addresses two puzzling findings related to mutations in galactocerebrosidase (GALC) that cause Krabbe disease (KD), a severe lysosomal storage disor
The number of people affected by spinal cord injuries (SCI) ranges from 2.5 to 4 million worldwide.
The initial trauma to the spinal cord is just the starting point for a cascade of endogenous events that will collectively determine the injury extension. .
In accordance with the trans-lamina cribrosa pressure difference theory, decreasing the trans-lamina cribrosa pressure difference can re-lieve glaucomatous opti
In adult mammals, axon regeneration after central nervous system injury is very poor, resulting in persistent functional loss. Enhancing the ability of axonal o
The mechanistic target of rapamycin (mTOR) is an intracellular protein kinase that functions as an energy and nutrient sensor in the cellular microenvironment o