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在固体培养体系中就麦穗离体时气温、甘露醇预处理及激素ABT各因子对大麦花药愈伤组织诱导及其分化的影响进行了研究。结果表明：预处理前供体所处气温高低对愈伤组织产量有一定的影响；甘露醇预处理效果优于低温处理；ZT和ABT连用对愈伤组织分化具有良好的促进作用，ABT最适浓度为0．3mg／L。
In the solid culture system, the effects of temperature, mannitol pretreatment and ABT factors on callus induction and differentiation of barley were studied. The results showed that the temperature of donor before pretreatment had a certain effect on the callus yield; mannitol pretreatment was better than the low temperature treatment; ZT and ABT had good promoting effect on callus differentiation, ABT optimum The concentration of 0.3mg / L.