目的:观察普罗布考(probucol)对糖基化终末产物(AGEs)作用后的心脏微血管内皮细胞iNOS表达的影响。方法:原代培养心脏微血管内皮细胞,AGEs(100 mg/L)体外模拟高糖环境,在probucol(5μmol/L,10μmol/L,20μmol/L)作用后,检测ROS、NO和iNOS变化情况。结果:与AGEs组比较,probucol组中ROS蛋白表达降低,NO生成增加,而iNOS蛋白表达降低,且显示有浓度依赖性。结论:probucol可能通过对抗氧化应激的途径,缓解AGEs引发的心脏微血管内皮功能障碍。 OBJECTIVE: To observe the effect of probucol on the expression of iNOS in cardiac microvascular endothelial cells induced by advanced glycation end products (AGEs). Methods: Cardiac microvascular endothelial cells were cultured in primary culture. AGEs (100 mg / L) were used to simulate high glucose in vitro. The changes of ROS, NO and iNOS were detected by probucol (5μmol / L, 10μmol / L, 20μmol / L) Results: Compared with AGEs group, the expression of ROS protein in probucol group was decreased, NO production was increased, and iNOS protein expression was decreased, and showed a concentration-dependent manner. CONCLUSIONS: probucol may relieve AGEs-induced cardiac microvascular endothelial dysfunction by counteracting oxidative stress.