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Moyamoya病的病因未明。除已知与遗传因素有关外,动脉壁的直接病理学改变和平滑肌细胞(SMC)增生可能在病变形成中起关键作用。内膜SMC的增生和游走可因内皮损伤而诱发。后者与血管内皮对流动血液中促细胞生长因子和细胞因子的反应性改变有关。本文旨在探讨SMC对生长因子和细胞因子反应性改变的影响以及是否参与了使Moyamoya病的内膜增厚。采用过去报道的方法,分离并培养12例Moyamoya病的SMC株(HMSMC)和8例其它疾病对照者的SMC株(HCSMC)。在微趋化腔内观察SMC的游走,用免疫过氧化酶技术测定DNA合成。 本组Moyamoya病患者中男女各6例,平均年龄9.6±3.8岁,发病年龄6.1±3.4岁。9例以TIA、2例以脑梗死、1例以脑出血为首发症状。对照组男3例,女5例,平均年龄与病例组无显著差异。在含有2％小牛血清(FBS)的微量基质(MEM)中,两组的细胞数无显著差异。血小板趋化生长因子BB(PDGF-BB)明
The cause of Moyamoya disease is unknown. In addition to being known to be associated with genetic factors, direct pathological changes of the arterial wall and proliferation of smooth muscle cells (SMCs) may play key roles in the development of the lesion. Endothelial SMC hyperplasia and migration can be induced by endothelial injury. The latter is associated with changes in the reactivity of vascular endothelial cells with circulating growth factors and cytokines in the bloodstream. This article aims to investigate the effect of SMC on growth factor and cytokine reactivity changes and whether they are involved in thickening of the intima of Moyamoya disease. Twelve MMC strains of Moyamoya disease (HMSMC) and eight other SMC strains (HCSMC) of disease control were isolated and cultured using the previously reported method. SMC migration was observed in the micrometastasis chamber and DNA synthesis was measured by the immunoperoxidase technique. The group of 6 patients with Moyamoya disease in men and women, with an average age of 9.6 ± 3.8 years, the onset age of 6.1 ± 3.4 years. 9 cases of TIA, 2 cases of cerebral infarction, 1 case of cerebral hemorrhage as the first symptom. Control group, 3 males and 5 females, the average age and the case group no significant difference. There was no significant difference in the number of cells in both groups in micro-matrix (MEM) containing 2% fetal bovine serum (FBS). Platelet chemotactic growth factor BB (PDGF-BB) Ming