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淋巴细胞是有机体内功能极为复杂而形态相似的一个细胞系,它们是参与免疫反应的重要的细胞成分,并与自身免疫病的发生有密切关系。根据细胞膜表面有不同的表面抗原、受体和免疫球蛋白以及它们在免疫反应中的功能不同,可以把淋巴细胞分为T、B、K和NK细胞以及不同的亚群。但现有的形态学染色方法和显微镜技术尚不能有效地区分它们。我们用自己建立的淋巴细胞悬浮染色方法,以一种荧光色素硫代黄素显示了十种荧光颜色不同的淋巴细胞,并结合免疫学手段进一步证明了在免疫反应过程中,其中四种淋巴细胞(桔红色荧光核黄色荧光胞质,灰黄色荧光核淡蓝绿色荧光胞质,深蓝色荧光核鲜蓝色荧光胞质和蓝色荧光核淡蓝色荧光胞质的淋巴细胞)可以分化为与它们荧光颜色相同的浆细胞,它们是B细胞。有关其它六种荧光颜色的淋巴细胞类型有待进一步探讨。此外,对淋巴细胞的分类和关于不同荧光颜色的淋巴细胞是不同类型,还是淋巴细胞的不同的分化阶段,或者是淋巴细胞的不同功能状态,以及浆细胞的分化过程进行了讨论。
Lymphocytes are extremely complex and morphologically similar cells in the body. They are important cellular components involved in the immune response and are closely related to the development of autoimmune diseases. Lymphocytes can be divided into T, B, K, and NK cells, as well as different subsets, based on the different surface antigens, receptors and immunoglobulins on the surface of the cell membrane and their different functions in the immune response. However, the existing morphological staining methods and microscopic techniques have not been able to effectively distinguish them. We use our own established lymphocyte suspension staining method, with a fluorescent dye thioflavin shows ten different fluorescent color lymphocytes, combined with immunological means further evidence that in the immune response, in which four kinds of lymphocytes (Orange-fluorescent nuclear yellow fluorescent cytoplasm, gray-yellow fluorescent nuclear light blue green fluorescent cytoplasm, dark blue fluorescent nuclear blue fluorescent cytoplasm and blue fluorescent nuclear light blue fluorescent cytoplasmic lymphocytes) can differentiate into They have the same fluorescent color of plasma cells, which are B cells. The other six types of fluorescent color lymphocytes to be further explored. In addition, the classification of lymphocytes and different types of lymphocytes with different fluorescent colors, different stages of lymphocyte differentiation, or different functional states of lymphocytes, and the differentiation process of plasma cells are discussed.