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Fish skin collagen hydrolysates(FSCH) were prepared from walleye pollock(Theragra chalcogramma) using a mixture of enzymes,namely trypsin and flavourzyme. The degree of hydrolysis of the skin collagen was 27.3%. FSCH was mainly composed of low-molecular-weight peptides and the relative proportion of <1 000Da fraction was 70.6%. Free radical and oxygen species scavenging activities of FSCH were investigated in four model systems,including diphenylpicrylhy-drazyl radical(DPPH),superoxide anion radical,hydroxyl radical and hydrogen peroxide model,and compared with that of a native antioxidant,reduced glutathione(GSH). FSCH was also evaluated by water-absorbing and water-holding capacity. The results showed that FSCH was able to scavenge free radical and oxygen species significantly and to enhance water-absorbing and water-holding capacity remarkably. Therefore,FSCH may have potential applications in the medicine and food industries.
The degree of hydrolysis of the skin collagen was 27.3%. FSCH was mainly composed of low-molecular-weight peptides Free radical and oxygen species scavenging activities of FSCH were investigated in four model systems, including diphenylpicrylhy-drazyl radical (DPPH), superoxide anion radical, hydroxyl radical and hydrogen peroxide model, and compared with that of a native antioxidant, reduced glutathione (GSH). FSCH was also evaluated by water-absorbing and water-holding capacity. The results was that FSCH was able to scavenge free radical and oxygen species significant and to enhance water-absorbing and Therefore, FSCH may have potential applications in the medicine and food industries.