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目的 探讨氧自由基 H2 O2 介导血管内皮细胞与白细胞粘附以及粘附的机理。方法 实验共分三组。 组 :H2 O2 2 5 0 UM刺激培养至对数生长期的人脐静脉内皮细胞 0 .5、1、2、3和 4小时 ,分别于刺激各时点加入 10 5个白细胞 ,共同孵育 2 0分后 ,镜下计数粘附到内皮细胞上的白细胞数。 组 :抗选择素 P的单克隆抗体与 H2 O2 共同作用内皮细胞 ,刺激时间与观察指标同 组。 组 :免疫细胞化学技术分析受 H2 O2 刺激内皮细胞膜粘附分子—选择素 P的表达。结果 组刺激各时点实验值同对照值比有显著性差异 (P<0 .0 1)。 组实验值同 组相同时点实验值比有显著性差异 , 组内皮细胞受 H2 O2 刺激细胞膜可表达选择素 P。结论 H2 O2 诱导内皮细胞表达选择素 P,后者介导了H2 O2 诱导的血管内皮细胞与白细胞粘附。
Objective To investigate the mechanism of oxygen free radical H2O2-mediated adhesion and adhesion of vascular endothelial cells and leukocytes. Methods The experiment is divided into three groups. Group: Human umbilical vein endothelial cells were stimulated at 0.5, 0.5, 1, 2, 3 and 4 h in logarithmic growth phase by H2 O2 2 0 0 UM, and 10 5 leukocytes were added at each time point to stimulate 20 Afterwards, the number of leukocytes adhered to endothelial cells was counted microscopically. Group: Monoclonal antibodies against selectin P interacted with H2O2 endothelial cells in the same group as stimuli. Group: Immunocytochemistry was used to analyze the expression of E-P, a membrane-bound adhesion molecule, to endothelial cells stimulated by H2O2. Results The experimental group at each time point was significantly different from the control group (P <0.01). The experimental group with the same experimental group at the same time the value was significantly different, endothelial cell H2O2-stimulated cell membrane expression of selectin P. Conclusion H2O2-induced endothelial cell expression of selectin P, which mediates H2O2-induced endothelial cell adhesion to leukocytes.