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目的:探讨清风胶囊对反复吗啡依赖和戒断引起的大鼠焦虑行为和脑内单胺递质的影响。方法:复制吗啡依赖大鼠成瘾和催促戒断后焦虑模型。将大鼠随机分空白对照组、吗啡模型组、清风胶囊低、中、高剂量组和阳性药地西泮对照组。清风胶囊低、中、高组给药剂量分别为0.5,1.0,2.0 g/kg,连续给药5天。观察动物焦虑行为的改变,并采用荧光分光光度计检测受试大鼠下丘脑单胺类神经递质去甲肾上腺素(NE)、5-羟色胺(5-HT)和多巴胺(DA)的含量。结果:清风胶囊0.5g/kg,1.0g/kg,2.0 g/kg三剂量均能明显缓解吗啡依赖大鼠由于反复躯体依赖和戒断所导致的焦虑行为,并显著减少大鼠下丘脑单胺类神经递质NE、5-HT和DA的含量。清风胶囊0.5,1.0,2.0 g/kg三剂量对大鼠焦虑行为的抑制作用与地西泮比较无明显差异,三剂量组之间亦无明显差异。结论:清风胶囊0.5g/kg,1.0g/kg,2.0 g/kg三剂量均对反复吗啡依赖大鼠戒断后的焦虑行为有明显的治疗效果。
Objective: To investigate the effect of Qingfeng capsule on anxiety behavior and monoamine neurotransmitter in rats induced by repeated morphine dependence and withdrawal. METHODS: Coping morphine-dependent rats were induced by addiction and reminded of withdrawal anxiety model. The rats were randomly divided into blank control group, morphine model group, Qingfeng capsule low, medium and high dose group and positive drug diazepam control group. Qingfeng capsule low, middle and high doses of 0.5,1.0,2.0 g / kg, continuous administration of 5 days. The changes of anxiety behaviors were observed. The levels of monoamine neurotransmitters, norepinephrine (NE), serotonin (5-HT) and dopamine (DA) in the hypothalamus were measured by fluorescence spectrophotometer. Results: The doses of 0.5g / kg, 1.0g / kg and 2.0g / kg of Qingfeng Capsule could relieve the anxiety caused by repeated physical dependence and withdrawal in morphine dependent rats and significantly reduce the levels of monoamine Contents of neurotransmitters NE, 5-HT and DA. There was no significant difference between the three doses of 0.5, 1.0 and 2.0 g / kg of breeze capsule on anxiety behavior of rats and diazepam, no significant difference between the three dose groups. Conclusion: The three doses of 0.5g / kg, 1.0g / kg and 2.0g / kg of Qingfeng Capsule have significant therapeutic effect on anxiety after repeated withdrawal from morphine dependent rats.