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目的观察旋毛虫免疫核糖核酸(iRNA)对BALB/c小鼠体内SP2/0肿瘤细胞的抑制作用。方法以不同剂量旋毛虫肌幼虫可溶性抗原免疫ICR小鼠、家兔和山羊,ELISA检测抗体效价达1:1024的最低免疫剂量确定为最佳免疫剂量,同时取各实验动物肝、脾和淋巴结,制备旋毛虫iRNA,并进行鉴定。以不同剂量iRNA接种BALB/c小鼠,并在不同时间接种SP2/0肿瘤细胞(试验分为先荷瘤和后荷瘤进行)。在荷瘤20d时,处死小鼠,测量肿瘤体积和重量,并检测脾脏T淋巴细胞亚群的变化。结果旋毛虫肌幼虫可溶性抗原的最佳免疫剂量分别为:ICR小鼠:300μg/只;家兔:2mg/只;山羊:20mg/只。制备的旋毛虫iRNA各项指标均合格。各试验组小鼠肿瘤体积和重量均小于相应的对照组,且差异均有统计学意义;各试验组小鼠的CD3+、CD4+及CD4+/CD8+比值较相应的对照组均有不同程度的增高。在后荷瘤试验组中,1mg组抑瘤效果最好;在先荷瘤试验组中,4mg组抑瘤效果最好。结论旋毛虫iRNA对BALB/c小鼠体内SP2/0肿瘤细胞的生长具有较强的抑制作用。
Objective To observe the inhibitory effect of Trichinella spiralis immune RNA (RNAi) on SP2 / 0 tumor cells in BALB / c mice. Methods ICR mice, rabbits and goats were immunized with different doses of soluble antigen of Trichinella spiralis larvae. The lowest immunization dose with ELISA titer of 1: 1024 was determined as the best immunization dose by ELISA. At the same time, the liver, spleen and lymph nodes The Trichinella iRNA was prepared and identified. BALB / c mice were inoculated with different doses of iRNA and SP2 / 0 tumor cells were inoculated at different times (the experiment was divided into first tumor and second tumor). Mice were sacrificed 20 days after tumor formation, tumor volume and weight were measured, and the changes of T lymphocyte subsets in spleen were detected. Results The optimum immunological doses of soluble antigen of Trichinella spiralis larvae were as follows: ICR mice: 300μg / rabbit; rabbit: 2mg / rabbit; goat: 20mg / mouse. Trichinella prepared iRNA indicators were qualified. The tumor volume and weight of mice in each experimental group were smaller than the corresponding control group, and the differences were statistically significant; the ratio of CD3 +, CD4 + and CD4 + / CD8 + of the mice in each experimental group was increased to some extent compared with the corresponding control group. In the later tumor-bearing test group, 1mg group had the best anti-tumor effect; in the first tumor-bearing test group, 4mg group had the best anti-tumor effect. Conclusion Trichinella iRNA has a strong inhibitory effect on the growth of SP2 / 0 tumor cells in BALB / c mice.