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以CDC生物膜反应器模拟给水管网输配系统,探究管网水相、生物膜相和颗粒物相三相界面下6种典型抗生素抗性细菌的分布特征.反应器稳定运行30d后,出水余氯从0.66mg/L下降到0.26mg/L,出水浊度和颗粒物浓度分别从进水的0.08NTU和377counts/mL增高至0.86NTU和4151counts/mL,出水水质变差.进水中仅有红霉素和氨苄西林的抗性细菌的数量较高,分别为36和99CFU/100mL,出水中,链霉素和氨苄西林的抗性细菌数量最高,分别为432和155CFU/100mL,远远高于进水中抗性细菌数量.生物膜相中异养菌总数和细胞总数达到了4089CFU/cm~2和1.5×10~6cells/cm~2,链霉素和红霉素的抗性细菌数量较高,为3432和2508CFU/cm~2,其抗性细菌比例分别达到了83.9%和61.4%.颗粒物相中,氯霉素和氨苄西林的抗性细菌比例较高,都在45%左右.生物膜和颗粒物都会给细菌提供一个安全稳定的生长场所,使细菌能够抵抗残留消毒剂和部分抗生素的抑制作用,更易产生耐药性,对人体健康的威胁也更大.
The CDC biofilm reactor was used to simulate the distribution system of water supply pipelines and to explore the distribution characteristics of six typical antibiotic-resistant bacteria in the water phase, biofilm phase and particulate phase of the pipe network.After 30 days of stable operation of the reactor, Chlorine decreased from 0.66mg / L to 0.26mg / L, effluent turbidity and particulate matter concentration increased from 0.08NTU and 377counts / mL of influent to 0.86NTU and 4151counts / mL, respectively, The number of resistant bacteria of ampicillin and ampicillin were higher, which were 36 and 99 CFU / 100 mL, respectively. The highest number of resistant bacteria of streptomycin and ampicillin in the effluent were 432 and 155 CFU / 100 mL respectively, much higher than The number of resistant bacteria in influent reached 4089CFU / cm ~ 2 and 1.5 × 10 ~ 6cells / cm ~ 2 in the biofilm phase, and the number of resistant bacteria of streptomycin and erythromycin High, 3432 and 2508CFU / cm ~ 2, the proportion of resistant bacteria reached 83.9% and 61.4% respectively.The proportion of resistant bacteria of chloramphenicol and ampicillin was higher in the particulate phase, all about 45% Membrane and particulate matter will provide bacteria with a safe and stable place of growth, so bacteria can resist the residual disinfectant and part Inhibition of antibiotics, the more resistance, the threat to human health is greater.