论文部分内容阅读
目的 检测ＴＮＦ相关凋亡诱导配体（ＴＲＡＩＬ）的受体，在来源于血液系统、肝脏、肺脏和大肠的８个肿瘤细胞系中的表达，并探讨其意义。方法采用半定量ＲＴ－ＰＣＲ，对ＴＲＡＩＬ受体的表达进行半定量检测。结果 ＴＲＡＩＬ凋亡通路中，能够诱导凋亡反应的死亡受体ＤＲ４和ＤＲ５，在所检测的肿瘤细胞系中都有表达，其中ＤＲ５在所有肿瘤细胞系中的表达水平均显著高于ＤＲ４（Ｐ＜０．０５）。而能够竞争性阻断ＴＲＡＩＬ诱导的凋亡反应的诱骗受体ＤｃＲ１和ＤｃＲ２，在所有的肿瘤细胞中都呈低水平表达或不表达。结论 ＤＲ５可能在ＴＲＡＩＬ诱导凋亡的通路中发挥最重要的作用。ＴＲＡＩＬ死亡受体和诱骗受体在肿瘤细胞系中的表达具有差异性，这种差异性可在一定程度上解释不同细胞对ＴＲＡＩＬ诱导凋亡的敏感度。
Objective To detect the expression of TNF-related apoptosis-inducing ligand (TRAIL) receptors in eight tumor cell lines derived from hematological, liver, lung and large intestine and to explore its significance. Methods Semi-quantitative RT-PCR was used to detect the expression of TRAIL receptor. Results DR4 and DR5, the death receptors capable of inducing apoptosis in TRAIL apoptotic pathway, all expressed in the tumor cell lines tested. The expression level of DR5 in all tumor cell lines was significantly higher than that in DR4 (P <0.05). While the decoy receptors DcR1 and DcR2, which can competitively block the TRAIL-induced apoptotic response, were expressed at low level or not in all tumor cells. Conclusion DR5 may play a most important role in the pathway of apoptosis induced by TRAIL. The differences in the expression of TRAIL death receptors and decoy receptors in tumor cell lines may explain to some extent the sensitivity of different cells to TRAIL-induced apoptosis.