本文以雌三醇—C_6肟在低温下经行酶标记,得高活力的酶标记物。抗体包被于40孔塑料板上,进行免疫反应,后用3,3′,5,5′-四甲基联苯胺硫酸盐反应显色。标准曲线范围为0～400Pg,灵敏度为12pg,回收率96％,批间变异系数为14.7％,批内变异系数7.6％,并测了正常值,与放射免疫法比较,结果相似。 In this paper, estriol-C_6 oxime at low temperature by enzyme labeling, high-activity enzyme markers. The antibody was coated on a 40-well plastic plate and subjected to an immunological reaction, followed by visualization with 3,3 ’, 5,5’-tetramethylbenzidine sulfate. The calibration curves ranged from 0 to 400 Pg, with a sensitivity of 12 pg. The recovery rate was 96%. The coefficient of variation (CV) between batches was 14.7%. The variation coefficient within the batches was 7.6% and the normal values ​​were measured. The results were similar to those of radioimmunoassay.