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目的探讨短时授精的时间对常规IVF的受精率及胚胎发育情况的影响。方法将病人授精时间分为4h、6h两组,观察卵母细胞的受精情况及胚胎发育情况。结果两组受精数占获卵数的比率分别为71.1%、75.9%,两组比较差异有显著性意义。受精后正常受精的卵比率为78.4%、79.9%,两组比较差异无显著性意义。卵裂率分别为97.1%、94.5%,两组比较差异有显著性意义。胚胎发育D3优质胚胎率分别为59.9%、54.83%,两组比较差异无显著性意义。结论延长短时授精的时间可以增加受精率,并不能增加正常受精卵的比率,反而使卵裂率降低。
Objective To investigate the effects of short-term insemination (IVF) fertilization on IVF fertility and embryo development. Methods The time of insemination was divided into 4h and 6h groups to observe the oocyte fertilization and embryo development. Results The ratio of fertilization to the number of oocytes in two groups was 71.1% and 75.9%, respectively. There was significant difference between the two groups. Fertilized fertilized eggs ratio of 78.4%, 79.9%, no significant difference between the two groups. Cleavage rates were 97.1%, 94.5%, respectively, with significant differences between the two groups. The rate of embryo development D3 high quality embryos were 59.9% and 54.83%, respectively, with no significant difference between the two groups. Conclusions Prolonging the time of short-term insemination can increase the fertilization rate, and can not increase the ratio of normal fertilized eggs, but reduce the cleavage rate.