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用非酶手工显微解剖法分离小麦（ＴｒｉｔｉｃｕｍａｅｓｔｉｖｕｍＬ．）合子与幼胚。应用金霉素（ＣＴＣ）和氟奋乃静（ＦＰＺ）荧光探针，观察了合子与胚胎发育过程中膜钙和活化钙调素的分布。合子原生质体ＣＴＣ荧光呈极性分布。二细胞至十几个细胞原胚时期各细胞间的ＣＴＣ和ＦＰＺ荧光均匀分布。幼小梨形胚胚体ＣＴＣ和ＦＰＺ荧光稍强于胚柄。接近分化的梨形胚ＣＴＣ荧光仅位于胚体与胚柄之间的几层细胞和胚体腹侧中部的几个细胞中。胚芽刚分化时，胚基部ＣＴＣ荧光较强，ＦＰＺ荧光则仅出现在胚基部。讨论了合子分离技术和膜钙与钙调素（ＣａＭ）在胚胎发育中的可能作用。
Wheat (Triticum aestivum L.) zygotes and immature embryos were isolated by non-enzymatic manual microdissection. The distribution of membrane calcium and activated calmodulin in the process of zygote and embryo development was observed by fluorescence probe of chlortetracycline (CTC) and fluphenazine (FPZ). CTC protoplast CTC fluorescence showed a polar distribution. Two cells to a dozen cells during the embryo of CTC and FPZ fluorescence evenly between the cells. Young pear embryoid body CTC and FPZ fluorescence slightly stronger than the embryo. Nearly differentiated pear-shaped embryos CTC fluorescence is located in only a few layers of cells between the embryoid body and the embryoid and several cells in the ventral midbrain of the embryo. Just germ bud differentiation, embryo base CTC fluorescence is strong, FPZ fluorescence is only found in the embryo base. The possible roles of zygotic separation and membrane calcium and calmodulin (CaM) in embryonic development are discussed.