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作者采用生物素－亲和素体系及化学发光增强法测定血清中庆大霉素含量。以微晶纤维素－羊抗兔抗体结合兔抗庆大霉素，再由待测庆大霉素与生物素化庆大霉素竞争，分离后沉淀中加入ＨＲＰ－亲和素，洗涤后测化学发光强度。结果测得血清中庆大霉素双对数标准曲线的检出限范围为６３ｎｇ～７８ｎｇ／ｔｕｂｅ，｜ｒ｜＝０．９９１±０．００６，平均回收率为１０３％。
The authors used biotin-avidin system and chemiluminescence enhancement method for the determination of gentamicin in serum. Rabbit gentamicin was conjugated with microcrystalline cellulose-goat anti-rabbit antibody and then competed with the biotinylated gentamicin for gentamicin to be tested. HRP-avidin was added into the precipitate after separation, washed and then tested Chemiluminescence intensity. Results The detection limit of gentamycin double logarithmic standard curve in serum ranged from 63 ng to 78 ng / tube and | r | = 0.991 ± 0.006 with an average recovery of 103%.