CRISPR/Cas system, originally developed as genetic editing tool, also shows great potentials for nucleotide detection.A recent study published in Molecular Cell (Freije et al., 2019)developed a Cas13a-based CARVER (Cas13-assisted restriction of viral expression and readout) to detect RNA viruses such as lymphocytic choriomeningitis, influenza A and vesicular stomatitis, which provided a potential expanded application for the detection of a broad range of viral nucleotides in disease diagnosis.