目的:探讨梓醇对缺血再灌注大鼠脑损伤后的保护作用。方法:采用传统大脑中动脉阻塞(MCAO)方法制备大鼠局灶性缺血模型,根据随机数字表法将SD大鼠分为MCAO组、对照组(vehicle组)及梓醇处理组(catalpol组),缺血再灌注48 h后观察各组大鼠神经功能学评分和脑梗死容积。分别于术前、术后6 h、24 h、48 h取大鼠脑组织样本,检测匀浆中谷胱甘肽过氧化物酶(GSH-PX)和丙二醛(MDA)的变化情况。结果:与vehicle组和MCAO组相比,catalpol处理组神经功能学评分降低(P<0.05);其梗死容积较小(P<0.05)。组织匀浆结果显示catalpol处理组脑匀浆中GSH-PX活力升高,MDA含量下降(P<0.05)。结论:梓醇可能通过降低脑内自由基水平、控制脂质过氧化程度,对缺血再灌注引起的大鼠脑损伤产生神经保护作用。 Objective: To investigate the protective effect of catalpol on brain injury after ischemia-reperfusion in rats. Methods: The focal cerebral ischemia model was induced by the middle cerebral artery occlusion (MCAO). The SD rats were divided into MCAO group, vehicle group and catalpol group according to random number table ), Neurological score and cerebral infarction volume of rats in each group were observed 48 hours after ischemia-reperfusion. Brain tissue samples of rats were taken before operation, 6 h, 24 h and 48 h after operation respectively to detect the changes of glutathione peroxidase (GSH-PX) and malondialdehyde (MDA). Results: Compared with the vehicle group and the MCAO group, the neurological score of the catalpol group was decreased (P <0.05) and the infarct volume was smaller (P <0.05). Tissue homogenization results showed that the activity of GSH-PX in the brain homogenate of catalpol treatment group increased and MDA content decreased (P <0.05). Conclusion: Catalpol may have a neuroprotective effect on brain injury induced by ischemia-reperfusion in rats by decreasing free radicals in the brain and controlling lipid peroxidation.