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为探讨核孔蛋白88(nucleoporin 88,Nup88)在胃腺癌组织中的表达及对胃腺癌SGC-7901细胞增殖、凋亡的影响研究,用Western blotting检测收集胃腺癌组织中的Nup88。通过细胞转染siRNA Nup88和siRNA control抑制胃腺癌细胞中Nup88的表达。MTT检测转染后细胞增殖情况。流式细胞术检测转染后细胞凋亡情况。Western blotting检测细胞中NOTCH信号通路相关蛋白NICD1、NICD2、HES1和Cleaved Caspase-3水平。胃腺癌细胞与NOTCH信号通路抑制剂S2188作用后,检测细胞增殖和凋亡情况。结果显示Nup88在胃腺癌组织中的表达水平明显高于癌旁组织(P<0.01)。siRNA Nup88组细胞中Nup88水平明显低于siRNA control组(P<0.01)。siRNA Nup88组细胞存活率明显低于siRNA control组(P<0.01)。siRNA Nup88组细胞凋亡率明显高于siRNA control组(P<0.01)。siRNA Nup88组细胞中NICD1、NICD2、HES1水平明显低于siRNA control组(P<0.01)。siRNA Nup88组细胞中Cleaved Caspase-3水平明显高于siRNA control组(P<0.01)。抑制剂作用后的细胞增殖凋亡趋势与siRNA Nup88组一致。由此,Nup88在胃腺癌组织中表达上调。沉默Nup88后胃腺癌细胞增殖受到抑制,凋亡增多,作用机制与NOTCH信号通路有关。
To investigate the expression of nucleoporin 88 (Nup88) in gastric adenocarcinoma and its effect on the proliferation and apoptosis of gastric adenocarcinoma SGC-7901 cells, we detected the expression of Nup88 in gastric adenocarcinoma by Western blotting. Inhibition of Nup88 expression in gastric adenocarcinoma cells by siRNA transfection of siRNA Nup88 and siRNA control. MTT detection of cell proliferation after transfection. Flow cytometry was used to detect apoptosis after transfection. The levels of NICD1, NICD2, HES1 and Cleaved Caspase-3 in NOTCH signaling pathway were detected by Western blotting. Gastric adenocarcinoma cells and NOTCH signal pathway inhibitor S2188 role in detecting cell proliferation and apoptosis. The results showed that the expression of Nup88 in gastric adenocarcinoma was significantly higher than that in paracancerous tissues (P <0.01). The level of Nup88 in siRNA Nup88 group was significantly lower than that in siRNA control group (P <0.01). The survival rate of siRNA Nup88 group was significantly lower than that of siRNA control group (P <0.01). The apoptosis rate of siRNA Nup88 group was significantly higher than that of siRNA control group (P <0.01). The level of NICD1, NICD2 and HES1 in siRNA Nup88 group was significantly lower than that in siRNA control group (P <0.01). The level of Cleaved Caspase-3 in siRNA Nup88 group was significantly higher than that in siRNA control group (P <0.01). Inhibitory effect of cell proliferation and apoptosis trends and siRNA Nup88 group. As a result, Nup88 is up-regulated in gastric adenocarcinoma. After silencing Nup88, the proliferation of gastric adenocarcinoma cells was inhibited and the apoptosis increased. The mechanism of action was related to NOTCH signaling pathway.