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目的 探索法医临床学评定广泛软组织挫伤程度量化指标。方法 采用改良的咖啡因法(J-G法)、放射免疫法分别检测广泛软组织挫伤后的人和大鼠血液中的胆红素(Bil)和肌红蛋白(Mb)浓度。结果 大鼠挫伤面积为6％、18％、30％,其血清胆红素浓度分别为4.86±0.4648、7.05±0.2915、8.52±0.1549μmol/1;肌红蛋白浓度分别为16.9140±0.9803、23.3435±1.3349、31.2490±1.8714ng/ml。二者经统计学分析P<0.001,均有显著性差异。结论测定血液中胆红素、肌红蛋白浓度有望成为法医临床学准确评估广泛软组织损伤程度的可靠量化指标。
Objective To explore forensic clinical evaluation of a wide range of quantitative indicators of soft tissue contusion. Methods The concentrations of Bil and Mb in human and rat after extensive soft tissue contusion were detected by modified method of Caffeine (J-G) and radioimmunoassay. Results The area of contusion in rats was 6%, 18% and 30%, respectively. The serum bilirubin concentrations were 4.86 ± 0.4648, 7.05 ± 0.2915 and 8.52 ± 0.1549μmol / 1, respectively. The myoglobin concentrations were 16.9140 ± 0.9803 and 23.3435 ± 1.3349, 31.2490 ± 1.8714 ng / ml. Both the statistical analysis of P <0.001, there were significant differences. Conclusion The determination of bilirubin and myoglobin concentrations in the blood is expected to become a reliable quantitative index for forensic clinicians to accurately assess the extent of extensive soft tissue injury.