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目的:检测卵巢癌组织中即刻早期反应基因X-1(IEX-1)和硝基酪氨酸(Nitro tyrosine,NT)的表达,探讨IEX-1与NT在卵巢癌发生发展中的作用。方法:选取郑州大学附属医院妇产科2007年1月～2008年10月104例卵巢肿瘤蜡块,其中良性卵巢肿瘤20例、交界性瘤24例、恶性组织60例,采用免疫组织化学方法检测卵巢组织中IEX-1与NT的表达,并分析两者的相关性。结果:①IEX-1在卵巢癌组织中的表达率38.3%,明显低于良性及交界性卵巢肿瘤(P<0.01);NT在卵巢癌组织中的表达率76.0%,明显高于良性及交界性卵巢肿瘤(P<0.001);②卵巢癌组织中IEX-1和NT蛋白的表达均与FIGO分期、组织学分级相关(P<0.05),与患者年龄、肿瘤的组织学类型无关(P>0.05);③IEX-1蛋白的阳性表达和NT蛋白的阳性表达在卵巢癌组织中的表达呈负相关(r=-0.271,P<0.05)。结论:IEX-1和NT蛋白的表达变化可能与卵巢癌的发生发展有关。
Objective: To detect the expression of immediate early response gene X-1 (IEX-1) and nitrotyrosine (NT) in ovarian cancer and to explore the role of IEX-1 and NT in the development of ovarian cancer. Methods: Obstetrics and Gynecology of Affiliated Hospital of Zhengzhou University from January 2007 to October 2008, 104 cases of ovarian tumor paraffin blocks, of which 20 cases of benign ovarian tumors, borderline tumors in 24 cases, 60 cases of malignant, detected by immunohistochemistry The expression of IEX-1 and NT in ovarian tissue and the correlation between them were analyzed. Results: ① The expression of IEX-1 in ovarian cancer tissues was 38.3%, which was significantly lower than that in benign and borderline ovarian tumors (P <0.01). The expression rate of NT in ovarian cancer was 76.0%, which was significantly higher than that in benign and borderline (P <0.001). ②The expression of IEX-1 and NT protein in ovarian cancer was correlated with FIGO staging and histological grade (P <0.05), but not with age and tumor histological type (P> 0.05 ). (3) There was a negative correlation between the positive expression of IEX-1 protein and the positive expression of NT protein in ovarian cancer (r = -0.271, P <0.05). Conclusion: The expression changes of IEX-1 and NT proteins may be related to the occurrence and development of ovarian cancer.