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研究了植病生防菌成团泛菌（Ｐａｎｔｏｅａ ａｇｇｌｏｍｅｒａｎｓ）的电击转化条件。结果表明受体菌固体培养、生长到对数早期时的细胞转化效果最好；质粒ＤＮＡ分子量增加 １０倍，转化效率降低 １００倍；质粒ＤＮＡ浓度在０．０１～１μｇ／μＬ范围内其变化与转化频率呈正比，与转化效率成反比；从成团泛菌中提纯的质粒ＤＮＡ比从大肠杆菌中提纯的相同质粒ＤＮＡ转化成团泛菌的效率高出３０倍５最佳电击条件为场强１５ｋＶ／ｃｍ、电阻２００Ｑ、电容２５μＦ，在此条件下受体菌的存活率约为１０％。按给出的条件，每微克５ｋｂ大小的质粒ＤＮＡ可以稳定地得到１０~５～１０~８个转化子。
The electroporation conditions of Pantoea agglomerans were studied. The results showed that the cell transformation of recipient bacteria was best when grown in early logarithmic phase. The molecular weight of plasmid DNA increased 10-fold and the transformation efficiency decreased by 100-fold. The change of plasmid DNA concentration in the range of 0.01-1 μg / μL The conversion frequency is proportional to the conversion efficiency and is inversely proportional to; the plasmid DNA purified from Pantoea agglomerans 30 times higher than that of the same plasmid DNA purified from E. coli 5 the best shock conditions for the field strength 15kV / cm, resistance 200Q, capacitance 25μF, in this condition the survival rate of the recipient bacteria is about 10%. According to the conditions given, 10 ~ 5 ~ 10 ~ 8 transformants can be stably obtained with 5kb plasmid DNA per microgram.