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目的观察胰岛素治疗对长期高脂喂养的糖尿病(DM)大鼠B细胞分泌功能的影响。方法2003-08~2004-11对华中科技大学同济医学院附属协和医院内分泌实验室的DM大鼠随机分为糖尿病正常饮食组(DN,n=10)、糖尿病高脂饮食组(DH,n=10)、糖尿病高脂饮食胰岛素治疗组(DHI,n=10)及正常Wistar大鼠正常饮食对照组(NC,n=10)。于胰岛素治疗后行口服葡萄糖耐量试验(OGTT)和胰岛素释放试验(IRT),并根据血糖和血胰岛素值计算糖负荷后胰岛素增值与血糖增值的比值(ΔI30/ΔG30)和修正的B细胞胰岛素分泌指数(MBCI)。留取空腹血浆测定游离脂肪酸(FFA)和甘油三酯(TG)。留取尾部胰腺组织测定胰腺内TG和FFA。结果与DN组和NC组相比,DH组血和胰腺内TG及FFA计量明显增多(分别为对DN组,P<0·01、P<0·01;对NC组,P<0·01、P<0·01;对DN组,P<0·01、P<0·05;对NC组,P<0·01、P<0·01),此外,ΔI30/ΔG30和MBCI显著降低(分别为对DN组,P<0·01、P<0·05,对NC组,P<0·01、P<0·01)。胰岛素治疗后,与DH组相比,胰腺内TG和FFA计量明显下降(分别为P<0·01、P<0·01),同时ΔI30/ΔG30、MCBI均得到明显改善(P<0·01、P<0·05)结论长期高脂饮食喂养可导致T2DM大鼠胰腺内FFA和TG沉积,并进一步损伤胰岛B细胞胰岛素的分泌;胰岛素治疗对高脂饮食所致的上述影响有一定的改善作用。
Objective To observe the effect of insulin therapy on B cell secretory function of long-term high-fat-fed diabetic (DM) rats. Methods From August 2003 to November 2004, DM rats in the endocrine laboratory of Union Hospital of Tongji Medical College of Huazhong University of Science and Technology were randomly divided into normal diet group (DN, n = 10), high fat diet group (DH, n = 10), diabetic hypercholesterolemia insulin treatment group (DHI, n = 10) and normal Wistar rats normal diet control group (NC, n = 10). The oral glucose tolerance test (OGTT) and insulin release test (IRT) were performed after insulin therapy and the ratio of insulin value to blood glucose increase (ΔI30 / ΔG30) and corrected B cell insulin secretion were calculated from the blood glucose and blood insulin values Index (MBCI). Fasting plasma samples were taken for the determination of free fatty acids (FFA) and triglycerides (TG). Tail pancreatic tissue was collected for the determination of TG and FFA in the pancreas. Results Compared with DN group and NC group, the levels of TG and FFA in blood and pancreas were significantly increased in DH group (DN group, P <0.01, P <0.01; in NC group, P <0.01 , P <0.01; DN group, P <0.01, P <0.05; NC group, P <0.01, P <0.01), in addition, ΔI30 / ΔG30 and MBCI were significantly decreased Respectively, for DN group, P <0.01, P <0.05, to NC group, P <0.01, P <0.01. After insulin treatment, the contents of TG and FFA in the pancreas decreased significantly compared with those in the DH group (P <0.01, P <0.01), and both ΔI30 / ΔG30 and MCBI were significantly improved (P <0.01 , P <0.05) Conclusion Long-term high-fat diet can lead to the deposition of FFA and TG in the pancreas of T2DM rats and further damage the insulin secretion of pancreatic islet B cells. Insulin treatment improves the above-mentioned effects caused by high fat diet effect.