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以7组红麻细胞质雄性不育系/保持系为材料,研究在12℃/6℃(昼/夜)低温胁迫下幼苗叶片相对电导率、丙二醛、脯氨酸、可溶性糖、可溶性蛋白含量及SOD和POD活性的变化,结果表明:持续低温胁迫下,红麻叶片的相对电导率、脯氨酸、可溶性糖、可溶性蛋白含量变化和SOD活性表现为先升高后降低;而POD活性和丙二醛含量则在胁迫9～15d后增加。利用隶属函数法和干物质重2种方法评定红麻材料间耐寒性强弱,发现相对电导率、可溶性蛋白和可溶性糖是反映红麻耐冷性差异的基本指标,上述2种方法比较红麻耐寒性强弱,其相关性达0.837**。供试的材料组合中耐寒性较强的材料分别是917A/B、722A/B和K03A/B。本研究结果可为今后进一步研究红麻耐冷防御措施和低温生理生化育种提供理论参考。
The effects of low temperature stress on the relative conductivity, malondialdehyde, proline, soluble sugar and soluble protein in leaves of seven kenaf cytoplasmic male sterile lines / maintainer lines were studied under 12 ℃ / 6 ℃ (day / night) Content and activities of SOD and POD in leaves of kenaf were studied. The results showed that the relative electric conductivity, the content of proline, soluble sugar, soluble protein and the activity of SOD in leaves of kenaf increased firstly and then decreased while the activities of POD And malondialdehyde content increased after 9 ~ 15d. The method of membership function and dry matter weight were used to assess the cold tolerance of kenaf materials. The relative conductivity, soluble protein and soluble sugar were found to be the basic indexes reflecting the differences in cold tolerance of kenaf. The above two methods were compared Sexual strength, the correlation of up to 0.837 **. The most cold-resistant materials in the tested combinations were 917A / B, 722A / B and K03A / B, respectively. The results of this study can provide theoretical reference for further research on cold tolerance and cold physiological and biochemical breeding of kenaf in the future.