用BSA (BulkedSegregantAnalysis)法获得了与核桃早实性状相关的RAPD标记OPB 0 890 0 。混合分离群体的混合样来自早实品种辽 1的自然授粉实生后代。用 2 2 0个随机引物扩增早实和晚实混合样 ,只有引物OPB 0 8(5’GTCCACACGG 3’)在早实混合样及其个体上扩增出了约 90 0bp的特异带OPB 0 890 0 ,而在晚实混合样及其个体上无此特异带。用OPB 0 8为引物 ,以 19个品种和 3个株系的DNA为模板进行扩增 ,特异带OPB 0 890 0 在 17个早实品种中的 12个个体上出现 ,而 5个晚实品种 (系 )上均无此带。 The RAPD marker OPB 0 890 0 related to the early fruiting characters of walnut was obtained by Bulked Segregation Analysis (BSA). Mixed mixed populations of mixed samples from the early varieties of Liao 1 natural pollination progeny. Two hundred and twenty random primers were used to amplify the early and late mixed samples. Only the primer OPB 0 8 (5’GTCCACACGG 3 ’) amplified about 90 0 bp of the specific band OPB 0 in early hybrid samples and individuals 890 0, but no specific bands were found in late-night mixed individuals and their individuals. OPB 0 8 was used as a primer to amplify the DNA of 19 varieties and 3 strains as templates. Specific band OPB 0 890 0 appeared on 12 individuals of 17 early-onset varieties and 5 late-onset varieties (Lines) are not on this band.