肿瘤细胞可以释放抑制各种细胞分裂的可溶性因子。通过外源凝集素(Lectin)、同种淋巴细胞和美洲商陆有丝分裂原诱导免疫球蛋白的产生,抑制淋巴细胞有丝分裂反应的能力证明,这些因子在体外显著的抑制活性,推测这些因子在决定人类肿瘤—宿主相互作用的后果中起重要作用,并为癌患者循环中免疫抑制因子的来源。作者报告了采用麦芽外源凝集素(Wheat-germ lectin)亲合层折法、PL-琼脂糖凝胶过沪和在1,2烷基硫酸钠(Sodium dodecylsulphat)存在时的聚丙烯凝胶电泳(PAGE-SDS)等大量连续分离的操作,实现了培养的肿瘤细胞上清液中有丝分裂抑制因子特征的研究。这些因子应用抑制PHA 刺激正常 Tumor cells can release soluble factors that inhibit various cell divisions. The ability to inhibit lymphocyte mitotic responses induced by lectins (Lectin), allogeneic lymphocytes, and pokeweed mitogens demonstrated that these factors have significant inhibitory activity in vitro, speculating that these factors are determining humans It plays an important role in the consequences of tumor-host interactions and is the source of immunosuppressive factors in the circulation of cancer patients. The authors report the use of a wheat-germ lectin affinity fold assay, PL-Sepharose gels, and polypropylene gel electrophoresis in the presence of sodium sodium sulphate (Sodium dodecylsulphat). A large number of continuous separation operations (PAGE-SDS) and the like have enabled the study of the characteristics of mitotic inhibitory factors in cultured tumor cell supernatants. The use of these factors inhibits normal PHA stimulation