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目的:研究口腔鳞癌(OSCC)中乙酰肝素酶(Heparanase,Hpa)mRNA的表达,探索其与微血管密度(mi-crovesseldensity,MVD)的关系,从而了解OSCC中Hpa在肿瘤血管生成中的作用,为OSCC的研究及治疗提供理论依据。方法:选择手术切除及活检的人OSCC标本64例,口腔正常黏膜10例,采用原位杂交技术检测HpamRNA的表达,用CD31抗体进行血管内皮的免疫组化染色,计数微血管密度。结果:HpamRNA在OSCC中的表达显著高于其在正常组织中的表达(P<0.05),HpamRNA阳性表达的OSCC组织中MVD显著高于阴性表达组(P<0.05),HpamRNA表达与淋巴结转移显著相关(P<0.05)。结论:Hpa能够促进OSCC的血管生成,参与了肿瘤淋巴结转移,可望成为口腔鳞癌抗血管生成治疗中的靶向因子。
OBJECTIVE: To study the expression of Heparanase (Hpa) mRNA in oral squamous cell carcinoma (OSCC) and to explore its relationship with microvessel density (MVD) so as to understand the role of Hpa in tumor angiogenesis in OSCC , To provide a theoretical basis for the study and treatment of OSCC. Methods: Sixty-four OSCC specimens were selected for surgical excision and biopsy, and 10 were normal mucosa. HpamRNA expression was detected by in situ hybridization. CD31 antibody was used for immunohistochemical staining of vascular endothelium and microvessel density was counted. Results: The expression of HpamRNA in OSCC was significantly higher than that in normal tissues (P <0.05). MVD in HpamRNA positive OSCC tissues was significantly higher than that in negative expression groups (P <0.05), and HpamRNA expression and lymph node metastasis were significant Related (P <0.05). Conclusion: Hpa can promote the angiogenesis of OSCC and participate in the tumor lymph node metastasis, which is expected to be a target factor in the anti-angiogenic therapy of oral squamous cell carcinoma.