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表皮生长因子(EGF)对乳腺等许多组织是一种有效的致有丝分裂因子。已证实其受体(EGF-γ)存在于乳腺癌等多种组织内.最近发现 EGF-γ的肽序列与已知的瘤基因相同.这说明 EGF-γ与细胞恶变能力之间有潜在联系。本研究旨在评价人乳腺癌EGF-γ的情况,并将其与雌激素受体(ER)、组织学和其他预后因素进行了比较。对61例原发乳腺癌和9例淋巴结转移瘤标本进行了 EGF-γ与 ER 测定.句化前在缓冲液中将组织碎丁,离心,蛋白浓度调至1～1.5mg/ml,加入~(125)Ⅰ标记的 EGF,在26℃孵育2小时,随后再离心,将结合与游离成分分离,计算其结果。应用饱和活性炭法检测 ER。
Epidermal growth factor (EGF) is an effective mitogenic factor in many tissues such as the mammary gland. It has been shown that its receptor (EGF-γ) is present in many tissues such as breast cancer. It was recently discovered that the peptide sequence of EGF-γ is the same as known tumor genes. This shows that there is a potential link between EGF-γ and the malignant ability of cells. . The aim of this study was to evaluate the condition of human breast cancer EGF-γ and to compare it with estrogen receptor (ER), histology and other prognostic factors. The EGF-γ and ER were measured in 61 cases of primary breast cancer and 9 cases of lymph node metastases. The tissue was diced in buffer and then centrifuged. The protein concentration was adjusted to 1 to 1.5 mg/ml. The (125) I-labeled EGF was incubated at 26°C for 2 hours and then centrifuged again to separate the binding from free components and the results were calculated. The saturated activated carbon method was used to detect ER.