我们改用全血法检测白细胞的杀菌功能,现将改进的方法报告如下。试剂和材料 1.Hank′s液;2.细胞营养液:0.5％水解乳蛋白,用无Ca~(++)、Mg~(++)的Hauk′s液配制;3.金黄色葡萄球菌悬液(2.5×10~7/ml)制备:将金黄色葡萄球菌接种于普通营养琼脂上,孵育过夜,刮取纯培养物,以营养液配成2.5×10~7/ml的细菌悬液;4.5％冰醋酸溶液; We use the whole blood test leukocyte bactericidal function, the improved method is now reported as follows. Reagents and materials 1. Hank’s solution; 2. Cell nutrient solution: 0.5% hydrolyzed milk protein, formulated with Hauk’s solution without Ca ~ (++), Mg ~ (++); 3. Staphylococcus aureus Suspension (2.5 × 10 ~ 7 / ml) Preparation: Staphylococcus aureus was inoculated on ordinary nutrition agar, incubated overnight, scraped pure culture, nutrient solution dubbed 2.5 × 10 ~ 7 / ml of bacterial suspension ; 4.5% glacial acetic acid solution;