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以金钱树叶片及叶茎为外植体进行离体培养研究,结果表明:用浓度为0.1%的升汞溶液对金钱树材料进行消毒,时间10～15min效果较佳;在以叶片和叶茎为外植体的愈伤组织诱导中,叶片较叶茎易产生愈伤组织;MS+6-BA2.0mg/L+NAA0.2mg/L的激素配比对诱导叶片愈伤组织的形成有利,诱导率高达74.1%;增殖培养基MS+6-BA2.0mg/L+NAA0.2mg/L最佳;不定芽在1/2MS+IBA1.0mg/L+NAA0.5mg/L的生根培养基上,生根最佳,生根率达86.2%,根多条、粗壮,植株长势良好、有活力;生根苗移栽在泥炭与椰糠2∶1的基质中,成活率较高。
The results showed that the cashmere material was disinfected with 0.1% mercuric chloride solution for 10-15 minutes, and the best effect was obtained when leaves and leaf stems were used as explants. In callus induction of explants, the leaves were easier to produce callus than the stems. The ratio of hormone with MS + 6-BA 2.0 mg / L + NAA 0.2 mg / L was beneficial to the induction of leaf callus formation, Induction rate was as high as 74.1%; proliferation medium MS + 6-BA2.0mg / L + NAA0.2mg / L was the best; adventitious buds were rooted on rooting medium with 1 / 2MS + IBA1.0mg / L + NAA0.5mg / L , Rooting best, rooting rate of 86.2%, many roots, stout, plants grow well, vitality; rooting seedlings transplanted in peat and coco noodles 2: 1 matrix, a higher survival rate.