论文部分内容阅读
作者选取SV40病毒的一个内含子缺失突变型(d1-2350,其剪接16SmRNA功能受损),用内切酶HpaⅡ在其基因组晚期区域(late region)处切开,生成粘性末端。同时使含小鼠β~(maj)珠蛋白基因的EcoR1片断在质粒pBR~(233)上无性扩增,取出质粒DNA后用HindⅡ切割,经凝胶电泳检出长为573个核苷酸的片段,它包括上述基因中的小内含子(内含子1)及其附近片断,但不含该基因的3’和
The authors selected an intron deletion mutant of the SV40 virus (d1-2350, impaired in its splicing 16S mRNA function), cleaving it with the endonuclease HpaII at its late genomic region to produce a cohesive end. At the same time, the EcoR1 fragment containing the mouse mastoglobin gene was amplified asexually on the plasmid pBR ~ (233). The plasmid DNA was extracted and cut with Hind II. The EcoR1 fragment was detected by gel electrophoresis and was 573 nucleotides in length Fragment, which includes the small intron (intron 1) and its vicinity in the above gene but does not contain the 3 ’and