论文部分内容阅读
有研究表明,正常人的胰岛素原样分子仅占血浆中免疫活性胰岛素样分子的10%～20%,但非胰岛素依赖型糖尿病(NIDDM)患者在空腹和口服葡萄糖以后循环中的胰岛素与胰岛素原的升高不成比例。故NIDDM的血浆胰岛素状态尚未确定。为了研究胰岛素缺乏在糖尿病发病学上的作用,作者用一种新的单克隆抗体双位点免疫放射分析(IRMA,与胰岛素原分子无交叉反应)法测定了NIDDM的血浆胰岛素,完整的胰岛素原和32-33胰岛素原片断,并与RIA法测定的血浆胰岛素浓度进行了比较。
Studies have shown that normal human insulin-like molecules account for only 10% to 20% of immune-active insulin-like molecules in plasma, but insulin and proinsulin in circulating non-insulin dependent diabetes mellitus (NIDDM) patients after fasting and oral glucose Increase is not proportional. Therefore, the plasma insulin status of NIDDM has not been determined. In order to study the role of insulin deficiency in the pathogenesis of diabetes, the authors used a novel monoclonal antibody dual site immunoradiometric assay (IRMA, no crossreactivity with proinsulin molecules) to determine plasma insulin, intact proinsulin And 32-33 proinsulin fragments were compared with plasma insulin concentrations measured by the RIA method.