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目的研究Lipofectamine2000转染psiRNa-STAT3质粒对4T1细胞侵袭及凋亡的作用。方法 Transwell小室检测psiRNa-STAT3质粒对乳腺癌细胞侵袭能力影响,Western-blot检测Cyclin-D1、Caspase-3蛋白表达水平。结果 Mock组与Scramble组穿膜细胞数比较差异无统计学意义(P>0.05),psiRNa-STAT3组穿膜细胞数与Scramble组比较差异有统计学意义(P<0.05);western-blot检测Scramble组Cyclin-D1、Caspase-3蛋白表达水平与Mock对照组比较差异无统计学意义(P>0.05)。psiRNa-STAT3组Caspase-3及Cyclin-D1蛋白表达量与Scramble组比较差异有统计学意义(P<0.05)。结论 psiRNa-STAT3质粒可能通过下调Caspase-3、Cyclin-D1蛋白表达从而抑制乳腺癌细胞侵袭能力,诱导细胞凋亡。
Objective To investigate the effect of Lipofectamine 2000 transfected psiRNa-STAT3 plasmid on invasion and apoptosis of 4T1 cells. Methods Transwell chamber was used to detect the influence of psiRNa-STAT3 on invasion ability of breast cancer cells. Western-blot was used to detect the protein expression of Cyclin-D1 and Caspase-3. Results The numbers of transmembrane cells in Mock group and Scramble group were not significantly different (P> 0.05), and the numbers of transmembrane cells in psiRNa-STAT3 group were significantly different from those in Scramble group (P <0.05) The expression of Cyclin-D1 and Caspase-3 protein in Mock control group had no significant difference (P> 0.05). The expression of Caspase-3 and Cyclin-D1 in psiRNa-STAT3 group was significantly lower than that in Scramble group (P <0.05). Conclusion psiRNa-STAT3 plasmid may inhibit the invasion ability of breast cancer cells and induce apoptosis by down-regulating Caspase-3 and Cyclin-D1 protein expression.