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我们将高转移性肺腺癌细胞亚系Anip_(973)及其母系AGZY_(83-a)(无转移性)注入裸小鼠腹腔,待出现腹水后,分离癌细胞,测定其PI-PLC活性(并经转移试验证实Anip_(973)仍具有转移能力)。实验结果表明具有不同转移表型的Anip_(973)和AGZY_(83-a)细胞PI-PLC活性有明显差异,前者为后者的2.25—7.1倍。体外培养两种细胞,测定两者PI-PLC活性,两者比值随传代次数增加逐步缩小,甚至出现倒置。两种细胞培养液均显示PI-PLC活性,且Anip_(973)高于AGZY_(83-a)。不同转移表型肺腺癌细胞PI-PLC活性在不同条件下的变化截然不同,此变化可能和肺腺癌细胞的转移功能密切相关。推测PI-PLC可能将成为新的转移标记酶。
We injected the metastatic lung adenocarcinoma sub-lineage Anip_(973) and its maternal AGZY_(83-a) (without metastasis) into the abdominal cavity of nude mice. After ascites, the cancer cells were isolated and the PI-PLC activity was measured. (And through the transfer test confirmed that Anip_ (973) still has the ability to transfer). The experimental results showed that the PI-PLC activities of Anip_(973) and AGZY_(83-a) cells with different metastatic phenotypes were significantly different. The former was 2.25 to 7.1 times higher than the latter. Two kinds of cells were cultured in vitro and their PI-PLC activities were measured. The ratio of the two was gradually reduced with the number of passages and even reversed. Both cell culture media showed PI-PLC activity, and Anip_ (973) was higher than AGZY_ (83-a). The changes of PI-PLC activity in different metastatic phenotypes of lung adenocarcinoma cells are quite different under different conditions. This change may be closely related to the metastatic function of lung adenocarcinoma cells. It is speculated that PI-PLC may become a new transfer marker enzyme.