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Objective:To understandtheclinicalsignificanceof CD4 + naturalkillerT(NK-T)cellsin chronic hepatitisB virus(HBV)infection.Methods :Peripheralbloodmononuclearcells(PBMCs)fromindividualswith chronicHBVinfectionwereseparated routinely.AfterInductionwithIL-12/IL-2for12d,theproportionof CD4 + NK-Tcellsin peripheralbloodwas determinedby fluorescenceactivatedcellsorter(FACS)analysis,andthe cytotoxicityof peripheralbloodlymphocytes(PBLs)was testedwitha4 h 51 Cr releaseassay.Results:After IL-12/IL-2induction,theproportionof CD4 + NK-Tcellswas(18.1±4.20)%, (6.95±2.85)%and(1.50±1.30)%inthe healthycontrol,CAHandAsCrespectively.Thatintheperipheralbloodof chronicHBVinfectedindividualswas lowerthanthatin thehealthycontrol.CD8+NK-Tcellswas(2.70±1.10)%, (2.20±1.40)%and(3.10±0.70)%respectively.In vitro cytotoxicityassaysagainstWishcellsrevealedthatthePBLscytotoxicityreducedin chronic HBVinfectedindividuals(P<0.05),andthatin AsCgroupwas significantlylowerin comparisonwithCHBand healthycontrolgroups.Thecytotoxicityof CD4 + NK-TcellsagainstWishcellscouldbe abolishedby treatingPBLs witheitheranti-CD4Abor anti-CD56Ab andcomplement,andpartiallydepleted by anti-CD8Ab.Conclusion:Theabnormalcellularimmunefunctionof chronicHBVinfectedindividualsmaybe associatedwiththedeficiency of CD4 + NK-Tcells.
Objective: To understand the clinical significance of CD4 + natural killer T (NK-T) cells in chronic hepatitis B virus (HBV) infection. Methods: Peripheral blood mononuclear cells (PBMCs) from infants with chronic HBV infection regimen routinely. AfterInduction with IL-12 / IL- 2for12d, theproportionof CD4 + NK- Tcellsin peripheral bloodwas determined by fluorescence activated cell death ) analysis, and the cytotoxicity of peripheral blood lymphocytes (PBLs) was tested witha4h51Cr releaseassay. Results: Afterproportionof CD4 + NK-T cells were (18.1 ± 4.20)%, (6.95 ± 2.85)% and (2.70 ± 1.10)%, (2.20 ± 1.40)% and (3.10 ± 0.70)% respectively. In vitro cytotoxicity Assays for the ischemic heart shed light of the PBL cytotoxicity in chronic HBV infection infected individuals (P <0.05) 1.30)% inthe healthy control, CA andAsClycologically.Thatintheperipheralbloodof chronicHBVinfectedindividualswaslowerthanthatinhehealthycontrol.CD8 + NK- , andthatinAsCgroupwas significantlylowerin comparisonwit hCHB and healthy control groups. The cytotoxic ability of CD4 + NK-T cells in sthish cells can be abolished by treatingPBLs witheitheranti-CD4 Abor anti-CD56Ab and complex, and partially-mediated by anti-CD8Ab.Conclusion: The aberrant cellular immune function of chronic HBV in infected individuals with dysficiency of CD4 + NK-T cells.