论文部分内容阅读
[目的]探讨聚合酶链反应(PCR)在慢性结膜炎病因诊断中的应用意义.[方法]选择慢性感染性结膜炎36例(36眼)、慢性免疫性结膜炎24例(24眼)及正常人22例(22眼),2眼共患结膜炎者取较重的1侧.提取受试者结膜囊分泌物DNA,分成2份,分别加入细菌引物及腺病毒引物进行PCR扩增,观察凝胶电泳结果.对慢性感染性结膜炎患者同时行结膜囊细菌培养,并观察其结果.[结果]慢性感染性结膜炎细菌感染阳性率明显高于病毒感染阳性率(χ2=7.40,P<0.05);PCR检测结果与细菌培养结果比较,慢性感染性结膜炎细菌感染阳性率间差异无统计学意义(χ2=3.20,P>0.05);[结论]PCR检测慢性感染性结膜炎是可供选择的方法,它在鉴别诊断慢性结膜炎的发病原因中具有重要的临床意义.
[Objective] To explore the significance of polymerase chain reaction (PCR) in the etiological diagnosis of chronic conjunctivitis. [Methods] Thirty-six patients with chronic conjunctivitis (36 eyes) and 24 eyes with chronic conjunctivitis 22 cases of normal (22 eyes), 2 patients with conjunctivitis who take heavier 1 side.Extract the conjunctival secretions of the subjects were divided into two parts, respectively, by adding bacterial primers and adenovirus primers for PCR amplification, The results of gel electrophoresis were observed. The bacterial culture of conjunctival sac in patients with chronic infectious conjunctivitis was also observed. [Results] The positive rate of bacterial infection in chronic infectious conjunctivitis was significantly higher than that in viral infection (χ2 = 7.40, P <0.05). There was no significant difference in the positive rate of bacterial infection between chronic infectious conjunctivitis (χ2 = 3.20, P> 0.05) by PCR test and bacterial culture results. [Conclusion] PCR detection of chronic infectious conjunctivitis was Alternative methods, it has important clinical significance in the differential diagnosis of the pathogenesis of chronic conjunctivitis.